Surface plasmon resonance imaging of cell-substrate contacts with radially polarized beams
نویسندگان
چکیده
منابع مشابه
Imaging of cell/substrate contacts of living cells with surface plasmon resonance microscopy.
We have developed a new method for observing cell/substrate contacts of living cells in culture based on the optical excitation of surface plasmons. Surface plasmons are quanta of an electromagnetic wave that travel along the interface between a metal and a dielectric layer. The evanescent field associated with this excitation decays exponentially perpendicular to the interface, on the order of...
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The central theme of this thesis is the use of imaging Surface Plasmon Resonance (iSPR) as a tool in the characterization of surfaces with laterally varying properties. Within the scope of this work, an instrument for iSPR analysis was designed and built. SPR is a very sensitive technique for monitoring changes in optical properties in the immediate vicinity of a sensor surface, which is very u...
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We show how a passive image-rotating optical resonator can be used to convert a linearly polarised, lowest-order Gaussian beam into a radially polarized beam. The image and polarization rotation of the cavity removes the frequency degeneracy of the modes, making it possible to select the radially polarized mode by cavity tuning. With the addition of gain, the same cavity should operate as a rad...
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1 Micro and Nanotechnology Laboratory, 2 Institute for Genomic Biology, 3 Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana –Champaign, IL, 61801 USA ABSTRACT We have created a randomly distributed nanocone substrate on silicon and coated with silver for surface-plasmonenhanced fluorescence detection and cell imaging. We observed three dimensional fluorescenc...
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ژورنال
عنوان ژورنال: Optics Express
سال: 2008
ISSN: 1094-4087
DOI: 10.1364/oe.16.020734